Journal: Nature

Article Title: Human germinal centres engage memory and naïve B cells after influenza vaccination

doi: 10.1038/s41586-020-2711-0

Figure Lengend Snippet: a) Binding of group 1–binding mAbs generated from singly sorted PBs and GC B cells that overlapped clonally (purple) or did not overlap (red and blue) for PB and GC B cells, respectively, from participant 05 using an influenza virus protein microarray (IVPM). Scale bar is median fluorescence intensity. Vaccine strains in bold type; underlined strains circulated in humans in participants’ lifetimes. b) Percentages of mAbs that bound two or more HA strains from participants 04, 05, and 11 from GC clones that did not participate in the early PB response (blue) and from PB and shared clones (red). P -values from Fisher’s exact test. The number of mAbs is indicated in the middle of the charts. c) Polyclonal epitopes of Fabs from plasma at indicated timepoints from participants 04, 05, and 11 with HA from A/Michigan/45/2015. Epitopes were determined by 3D reconstructions and/or 2D class averages (images to bottom right of 3D reconstructions). HA proteins shown in grey; Fabs shown in multiple colors. d) Monoclonal and polyclonal epitopes of immune complexes with HA from A/Michigan/45/2015 and Fabs generated from the indicated GC mAbs (blue) and plasma pAbs (red). Fabs with dashed outlines have predicted epitopes due to limited particle representation. e) Protection of GC mAbs 1B05 and 2C09 in a mouse influenza virus challenge model. Mice received 5 mg/kg of the indicated mAb intraperitoneally 1 day before intranasal challenge with A/California/04/2009 E3 (H1N1), and were weighed daily; 7 mice were used for 1B05 and 1G01, 6 for 2C09 and isotype control, and 5 for uninfected. Error bars indicate mean ±SEM.

Article Snippet: Arrays were imaged and analyzed with a Vidia microarray scanner (Indevr) using an exposure time of 1000 ms to measure spot median fluorescence.

Techniques: Binding Assay, Generated, Virus, Microarray, Fluorescence, Clone Assay, Clinical Proteomics, Control

Journal: Scientific Reports

Article Title: Fabrication of a protein microarray by fluorous-fluorous interactions

doi: 10.1038/s41598-017-07571-4

Figure Lengend Snippet: Fluorous protein microarray for the simultaneous detection of multiple proteins. ( a ) MBP, GST, and anti-RCA 120 were printed on a fluorous slide. ( b ) Confirmation of the specific fluorous-fluorous interactions. Anti-RCA 120 , anti-RCA 120 pre-incubated with F tag -acid (compound 5), and anti-RCA 120 pre-incubated with F tag -BA (compound 2) solutions were spotted on a fluorous slide.

Article Snippet: The activity of fluorescent eGFP on the slide was directly measured with a NovaRay Microarray Scanner using a fluorescein isothiocyanate (FITC) filter.

Techniques: Microarray, Incubation

Journal: Glia

Article Title: Inhibition of inflammatory cells delays retinal degeneration in experimental retinal vein occlusion in mice

doi: 10.1002/glia.23739

Figure Lengend Snippet: Results from mouse cytokine antibody microarray for a selection of (a) proinflammatory and (b) anti‐inflammatory proteins. Significance levels: * p < .05, ** p < .01, *** p < .001, **** p < .0001; ♦: expression level below detection threshold (0.1 pg/ml)

Article Snippet: After washing and drying steps, a glass slide scanner with a Cy3 filter was used for fluorescence intensity detection (ScanRI Microarray Scanner, PerklinElmer, Inc., MA).

Techniques: Microarray, Selection, Expressing